Goat anti-cytokeratin 20 (aa48-60) Antibody

SKU: orb12296

Description

Goat polyclonal antibody to KRT20

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Application Notes	WB: Approx 49kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5kDa according to NP_061883.1). Recommended concentration: 0.01-0.03µg/ml.. IHC: Paraffin embedded Human Colon. Recommended concentration: 5µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	cytokeratin 20 (aa48-60)
Protein Sequence	NSRHTVNYGSDLT
Molecular Weight	48.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti CK20 antibody, anti CK-20 antibody, anti cytokeratin 20 antibody, anti cytokeratin-20 antibody, anti K20 antibody, anti keratin 20 antibody, anti keratin, type I cytoskeletal 20 antibody, anti keratin-20 antibody, anti KRT20 antibody, anti KRT21 antibody, anti MGC35423 antibody, anti OTTHUMP00000164518 antibody, anti protein IT antibody

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Goat anti-cytokeratin 20 (aa48-60) Antibody

Western blot analysis of Human Colon lysate using KRT20 antibody

Immunohistochemical staining of Human Colon using KRT20 antibody.

Quick Database Links

Gene Symbol

cytokeratin 20 (aa48-60)

NCBI Gene ID

54474

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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