Goat anti-Endothelial lipase Antibody

SKU: orb20030

Description

Goat polyclonal antibody to LIPG

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:16000, WB: 0.3-1 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 70kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al , J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. Epub 2007 Mar 13.; PMID: 17356047). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Endothelial lipase
Molecular Weight	56.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti endothelial lipase antibody, anti lipase, endothelial antibody, anti LIPG antibody, anti EDL antibody, anti EL antibody, anti PRO719 antibody, anti endothelial cell-derived lipase antibody, anti lipoprotein lipase H antibody

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Western blot analysis of Human Liver lysate using LIPG antibody

Quick Database Links

Gene Symbol

Endothelial lipase

NCBI Gene ID

9388

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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