Goat anti-ERG Antibody

SKU: orb19134

Description

Goat polyclonal antibody to ERG

Research Area

Cancer, Epigenetics, Stem Cells

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, WB
Dilution range	ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Prostate shows nucleolar staining in some of the epithelial cells. Recommended concentration, 10-20ug/ml.WB: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	ERG
Molecular Weight	53.8; 52.0; 54.6; 44.0
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody, anti- D030036I24Rik antibody, anti- Erg 3 antibody, anti- Erg antibody, anti- ERG/EWS fusion gene, included antibody, anti- ERG/FUS fusion gene, included antibody, anti- ERG/TMPSSR2 fusion gene, included antibody, anti- ERG_human antibody, anti- ERG1, included antibody, anti- ERG2, included antibody, anti- ets related antibody, anti- ETS-related gene antibody, anti- KCNH2 antibody, anti- Oncogene ERG antibody, anti- p55 antibody, anti- TMPRSS2/ERG fusion antibody, anti- transcriptional regulator ERG (transforming protein ERG) antibody, anti- Transcriptional regulator ERG antibody, anti- Transforming protein ERG antibody, anti- v ets avian erythroblastosis virus E26 oncogene antibody, anti- v ets avian erythroblastosis virus E26 oncogene related antibody, anti- v ets erythroblastosis virus E26 oncogene homolog antibody, anti- v ets erythroblastosis virus E26 oncogene like antibody, anti- v ets erythroblastosis virus E26 oncogene like isoform 2 antibody, anti- v-ets erythroblastosis virus E26 oncogene antibody, anti- v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody, anti- V-ets erythroblastosis virus E26 oncogene like (Avian) antibody, isoform CRA_e antibody

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Immunohistochemical staining of Human Prostate using ERG antibody

Western blot analysis of MOLT4 lysate using ERG antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using ERG antibody.

Immunofluorescence analysis of MCF7 cells using ERG antibody.

Quick Database Links

Gene Symbol

ERG

NCBI Gene ID

2078

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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