Frizzled 4 Antibody

SKU: orb19753

Description

Goat polyclonal antibody to FZD4

Research Area

Stem Cells

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:16000, WB: 0.3-1 μg/ml
Reactivity	Human
Predicted Reactivity	Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 60kDa band observed in Human Colon and Small Intestine (Ileum) lysates (calculated MW of 59.9kDa according to NP_036325.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Frizzled 4
Protein Sequence	DEEERRCDPIRIS
Molecular Weight	59.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti Frizzled 4 antibody, anti frizzled homolog 4 (Drosophila) antibody, anti FZD4 antibody, anti EVR1 antibody, anti FEVR antibody, anti FZD4S antibody, anti Fz-4 antibody, anti FzE4 antibody, anti GPCR antibody, anti MGC34390 antibody, anti WNT receptor frizzled-4 antibody, anti exudative vitreoretinopathy 1 (autosomal dominant Criswick-Schepens syndrome) antibody, anti frizzled 4 antibody

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orb19753 (1ug/ml) staining of Small Intestine (Ileum) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Frizzled 4

NCBI Gene ID

8322

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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