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Frizzled 7 Antibody
SKU: orb19747
Description
Research Area
Stem Cells
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | Frizzled 7 |
| Protein Sequence | RFYHRLSHSSKGET |
| Molecular Weight | 63.6 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti rizzled 7 antibody, anti frizzled homolog 7 (Drosophila) antibody, anti FZD7 antibody, anti FzE3 antibody, anti Frizzled, drosophila, homolog of, 7 antibody
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orb19747 (0.3ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19747 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Frizzled 7 Antibody (orb19747)
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