GAD1 (isoform GAD67) Antibody

SKU: orb20081

Description

Goat polyclonal antibody to GAD1

Research Area

Epigenetics, Neuroscience

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, FC, IF, WB
Dilution range	ELISA: 1:32000, WB: 0.5-1.5 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	GAD1 (isoform GAD67)
Molecular Weight	66.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti GAD1 antibody, anti glutamate decarboxylase 1 (brain, 67kDa) antibody, anti FLJ45882 antibody, anti GAD antibody, anti SCP antibody, anti OTTHUMP00000041055 antibody, anti glutamate decarboxylase 1 antibody, anti glutamate decarboxylase 1 (brain, 67kD) antibody, anti GAD67 antibody, anti GAD25 antibody

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orb20081 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb20081 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb20081 (1µg/ml) staining of Human Cerebellum (A) and Mouse Brain (B) and (0.3ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb20081 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Human Brain lysate using GAD1 antibody

Immunohistochemical staining of Human Cerebellum using GAD1 antibody

Quick Database Links

Gene Symbol

GAD1 (isoform GAD67)

NCBI Gene ID

2571

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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