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GFAP Antibody
SKU: orb19324
Description
Research Area
Epigenetics, Neuroscience, Stem Cells
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:128000, WB: 0.001-0.1 μg/ml |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Canine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | GFAP |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti GFAP antibody, anti glial fibrillary acidic protein antibody, anti FLJ45472 antibody, anti intermediate filament protein antibody
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orb19324 (0.001µg/ml) staining of Human Cerebellum (A), (0.1µg/ml) Mouse Brain (B) and (0.03µg/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Western blot analysis of Human Brain (Cerebellum) (A), Mouse (B) and Rat (C) Brain lysate using GFAP antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GFAP Antibody (orb19324)
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