GPX4 (Isoform a and c) Antibody

SKU: orb19310

Description

Goat polyclonal antibody to GPX4

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, IP, WB
Dilution Range	ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 0.3 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3ug/ml.WB: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	GPX4 (Isoform a and c)
Protein Sequence	EEPLVIEKDLPHY
Molecular Weight	22.1; 27.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti GPX4 antibody, anti glutathione peroxidase 4 (phospholipid hydroperoxidase) antibody, anti HGNC:4556 antibody, anti PHGPx antibody, anti snGPx antibody, anti glutathione peroxidase 4 antibody, anti phospholipid hydroperoxidase antibody, anti sperm nucleus glutathione peroxidase antibody

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0.03 μg/ml staining of Human (A), Mouse (B) and (0.01 μg/ml) Rat (C) Testes lysate. Detected by chemiluminescence.

0.1 μg/ml staining of Human (A) and (1 μg/ml) Rat (B) Kidney lysate. Detected by chemiluminescence.

4 μg/ml staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Quick Database Links

Gene Symbol

GPX4 (Isoform a and c)

NCBI Gene ID

2879

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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