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HDAC6 Antibody

SKU: orb154549

Description

Goat polyclonal antibody to HDAC6, which is a member of the histone deacetylase family, HD type 2 subfamily. It is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. It acts in microtubule-dependent cell motility via deacetylation of tubulin. HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network.

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC
Dilution RangeELISA: 1:32000, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
WB: Approx 150kDa band observed in nuclear lysates of cell lines NIH3T3, and HeLa (calculated MW of 131kDa according to NP_006035.2). Recommended concentration: 1-3 μg/ml.

Key Properties

ClonalityPolyclonal
TargetHDAC6
Protein SequenceAHQNKFGEDMPHPH
Molecular Weight131; 132.8
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti HDAC6 antibody, anti histone deacetylase 6 antibody, anti HD6 antibody, anti JM21 antibody, anti KIAA0901 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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