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Description
Research Area
Pharmacology & Drug Discovery
Images & Validation
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Item 1 of 3
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to Human NP_001354640.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | Histamine Receptor H2 |
| Protein Sequence | QEEKPLKLQVWSGTE |
| Molecular Weight | 44.5; 46.9 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti HRH2 antibody, anti histamine receptor H2 antibody, anti HGNC:5183 antibody, anti H2R antibody, anti gastric receptor 1 antibody, anti OTTHUMP00000161242 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Species
Zhuang, Qian-Xing et al. Histamine Excites Striatal Dopamine D1 and D2 Receptor-Expressing Neurons via Postsynaptic H1 and H2 Receptors Mol Neurobiol, 55, 8059-8070 (2018)
Reactivity
Rat
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