IGF2BP2 Antibody

SKU: orb19766

Description

Goat polyclonal antibody to IGF2BP2

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:16000, WB: 1-3 μg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	IGF2BP2
Molecular Weight	66.1; 61.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti IGF2BP2 antibody, anti insulin-like growth factor 2 mRNA binding protein 2 antibody, anti IMP-2 antibody, anti IMP2 antibody, anti VICKZ2 antibody, anti p62 antibody, anti IGF II mRNA binding protein 2 antibody, anti IGF-II mRNA-binding protein 2 antibody

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orb19766 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IGF2BP2 (RC205673) and probed with orb19766 (mock transfection in first lane), tested by Origene.

Quick Database Links

Gene Symbol

IGF2BP2

NCBI Gene ID

10644

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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