IMP1/ZBP1 Antibody

SKU: orb20564

Description

Goat polyclonal antibody to IGF2BP1

Research Area

Epigenetics

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3;). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	IMP1 / ZBP1
Protein Sequence	EKVFAEHKISYSGQ
Molecular Weight	63.5; 48.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti coding region determinant-binding protein antibody, anti CRDBP antibody, anti CRD-BP antibody, anti IGF II mRNA binding protein 1 antibody, anti IGF2 mRNA-binding protein 1 antibody, anti IMP1 antibody, anti IMP-1 antibody, anti insulin-like growth factor 2 mRNA binding protein 1 antibody, anti VICKZ family member 1 antibody, anti VICKZ1 antibody, anti ZBP1 antibody, anti zipcode-binding protein 1 antibody, anti IGF2BP1 antibody

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0.1 µg/ml staining of Caco-2 (A), (0.3 µg/ml) K562 (B), nuclear HepG2 (C) and (1 µg/ml) nuclear NIH3T3 (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

3.75 µg/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

IMP1 / ZBP1

NCBI Gene ID

10642

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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