KAP1/TRIM28 Antibody

SKU: orb18424

Description

Goat polyclonal antibody to TRIM28

Images & Validation

−

Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC
Dilution range	ELISA: 1:2000, IHC-P: 2-4 μg/ml
Reactivity	Human
Predicted Reactivity	Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899). Recommended concentration: 5ug/ml.WB: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1 μg/ml (Predicted MW approx 92kDa according to NP_005753).Experiment Notes: Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication). Read more...

Key Properties

−

Host	Goat
Clonality	Polyclonal
Target	KAP1 / TRIM28
Molecular Weight	88.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

−

anti TRIM28 antibody, anti KAP1 antibody, anti TF1B antibody, anti RNF96 antibody, anti TIF1B antibody, anti tripartite motif-containing 28 antibody, anti KRAB-associated protein 1 antibody, anti nuclear corepressor KAP-1 antibody, anti transcriptional intermediary factor 1-beta antibody, anti FLJ29029 antibody, anti KRAB-associated protein 1 antibody, anti tripartite motif-containing 28 protein antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

orb18424 staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Breast using KAP1 antibody

Immunohistochemical staining of Human Breast using TRIM28 antibody

Western blot analysis of HepG2 lysate using TRIM28 antibody

Quick Database Links

Gene Symbol

KAP1 / TRIM28

NCBI Gene ID

10155

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC

Immunohistochemistry

View Protocol

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols