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KCNQ1 Antibody

SKU: orb20163

Description

Goat polyclonal antibody to KCNQ1

Research Area

Epigenetics, Neuroscience

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:16000, WB: 1-3 μg/ml
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 60kDa band observed in Human Heart lysates (calculated MW of 61.5kDa according to NP_861463.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetKCNQ1
Protein SequenceEQLTVPRRGPDEGS
Molecular Weight74.7; 61.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti KCNQ1 antibody, anti potassium voltage-gated channel, KQT-like subfamily, member 1 antibody, anti ATFB1 antibody, anti FLJ26167 antibody, anti JLNS1 antibody, anti KCNA8 antibody, anti KCNA9 antibody, anti KVLQT1 antibody, anti Kv1.9 antibody, anti Kv7.1 antibody, anti LQT antibody, anti LQT1 antibody, anti RWS antibody, anti SQT2 antibody, anti WRS antibody, anti Jervell and Lange-Nielsen syndrome 1 antibody, anti kidney and cardiac voltage dependend K+ channel antibody, anti long (electrocardiographic) QT syndrome, Ward-Romano syndrome 1 antibody, anti slow delayed rectifier channel subunit antibody

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orb20163 (8 µg/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

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orb20163 Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

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orb20163 (1 µg/ml) staining of Human Heart lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

KCNQ1 Antibody (orb20163)

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Available Sizes

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100 μg
¥ 6,890.00
DispatchUsually dispatched within 5-10 working days
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