MDA5/IFIH1 Antibody

SKU: orb18521

Description

Goat polyclonal antibody to IFIH1

Research Area

Epigenetics, Immunology, Infectious Diseases

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, FC, IHC, WB
Dilution range	ELISA: 1:32000, IHC-P: 10ug/ml
Reactivity	Human, Monkey
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10ug/ml.WB: No signal obtained yet but low background observed in Human Placenta lysate at up to 1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	MDA5 / IFIH1
Molecular Weight	117
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti IFIH1 antibody, anti MDA5 antibody, anti MDA-5 antibody, anti Hlcd antibody, anti MGC133047 antibody, anti IDDM19 antibody, anti melanoma differentiation associated protein-5 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide antibody, anti interferon induced with helicase C domain 1 antibody, anti helicard antibody

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orb18521 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Quick Database Links

Gene Symbol

MDA5 / IFIH1

NCBI Gene ID

64135

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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