Myelin Protein zero Antibody

SKU: orb19823

Description

Goat polyclonal antibody to MPZ

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:32000, WB: 0.1-0.3 μg/ml
Reactivity	Rat
Predicted Reactivity	Canine, Human, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 28kDa band observed in Rat Spinal Cord lysates (calculated MW of 28.5kDa according to Human NP_000521.1 and of 27.7kDa according to Rat NP_058723.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Myelin Protein zero
Protein Sequence	DHSRSTKAVSEK
Molecular Weight	28.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti myelin protein zero antibody, anti myelin protein zero (Charcot-Marie-Tooth neuropathy 1B) antibody, anti MPZ antibody, anti CHM antibody, anti CMT1 antibody, anti CMT1B antibody, anti CMT2I antibody, anti CMT2J antibody, anti CMT4E antibody, anti CMTDI3 antibody, anti DSS antibody, anti HMSNIB antibody, anti MPP antibody, anti P0 antibody

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orb19823 (0.1ug/ml) staining of Rat Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Myelin Protein zero

NCBI Gene ID

4359

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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