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Description
Research Area
Metabolism Research
Images & Validation
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Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 75kDa band observed in lysates of cell lines HEK293 and NIH3T3 and in Rat Heart lysates, and approx. 80kDa in Human and Mouse Heart and in Human Skeletal Muscle lysates (calculated MW of 79.5kDa according to Human NP_004997.4 and Rat NP_001005550.1, 75.4kDa according to Human NP_001186910.1, and 79.8kDa according to Mouse NP_001153510.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | NDUFS1 |
| Protein Sequence | TEKSATYVNTEGR |
| Molecular Weight | 79.5 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti CI-75Kd antibody, anti complex I, mitochondrial respirato antibody, anti MGC26839 antibody, anti NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa antibody, anti NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) antibody, anti NADH-coenzyme Q reductase antibody, anti OTTHUMP00000206345 antibody, anti PRO1304 antibody, anti NDUFS1 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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