Neuroligin 1 Antibody

SKU: orb19055

Description

Goat polyclonal antibody to NLGN1

Research Area

Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF
Dilution range	ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 10 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Neuroligin 1
Molecular Weight	92
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti NLGN1 antibody, anti KIAA1070 antibody, anti MGC45115 antibody, anti neuroligin 1 antibody, anti OTTHUMP00000211275 antibody, anti OTTHUMP00000211276 antibody

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Immunofluorescence analysis of U251 cells using NLGN1 antibody

Immunofluorescence analysis of U2OS cells using NLGN1 antibody

Quick Database Links

Gene Symbol

Neuroligin 1

NCBI Gene ID

22871

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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