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NOTCH3 Antibody

SKU: orb19112

Description

Goat polyclonal antibody to NOTCH3

Research Area

Cardiovascular Disease, Developmental Biology, Epigenetics, Neuroscience

Images & Validation

Tested ApplicationsELISA, FC, IHC
Dilution RangeELISA: 1:16000, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetNOTCH3
Protein SequenceQLGPQPEVTPKRQ
Molecular Weight244
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti NOTCH3 antibody, anti Notch homolog 3 (Drosophila) antibody, anti HGNC:7883 antibody, anti CADASIL antibody, anti CASIL antibody, anti Notch (Drosophila) homolog 3 antibody, anti Notch homolog 3 antibody

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NOTCH3 Antibody (orb19112)

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100 μg
¥ 6,890.00
DispatchUsually dispatched within 5-10 working days
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