OCT4/POU5F1 Antibody

SKU: orb19274

Description

Goat polyclonal antibody to POU5F1

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:2000, WB: 0.5--2 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Canine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 38kDa band observed in Human Liver lysates (calculated MW of 38.6kDa according to NP_002692.2). Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	OCT4 / POU5F1
Protein Sequence	C-VTTLGSPMHSN
Molecular Weight	38.6; 30.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti POU5F1 antibody, anti OTF4 antibody, anti POU domain, class 5, transcription factor 1 antibody, anti HGNC:9221 antibody, anti MGC22487 antibody, anti OCT3 antibody, anti OTF3 antibody, anti POU-type homeodomain-containing DNA-binding protein antibody, anti octamer-binding transcription factor-3 antibody, anti POU class 5 homeobox 1 antibody, anti DADB-104B20.2 antibody, anti OCT4 antibody, anti POU domain, class 5, transcription factor 1 antibody

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orb19274 (2µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19274 (0.5µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

OCT4 / POU5F1

NCBI Gene ID

5460

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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