OX1R and OX2R Antibody

SKU: orb19864

Description

Goat polyclonal antibody to Orexin Receptor

Research Area

Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:32000, WB: 0.3-1 μg/ml
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	OX1R and OX2R
Molecular Weight	47.5; 50.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti-HCRTR 1 antibody, anti-HCRTR1 antibody, anti-Hypocretin receptor type 1 antibody, anti-Orexin receptor type 1 antibody, anti-Ox-1-R antibody, anti-Ox1-R antibody, anti-Ox1r antibody, anti-OX1R_HUMAN antibody

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Western blot analysis of Human Frontal Cortex lysate using HCRTR1 antibody

Western blot analysis of Mouse Brain lysate using HCRTR1 antibody

Quick Database Links

Gene Symbol

OX1R and OX2R

NCBI Gene ID

3061, 3062

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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