Prefoldin Antibody

SKU: orb18833

Description

Goat polyclonal to Prefoldin

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:32000, WB: 1-3 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments (at 0.2 μg/ml) gave a strong band at approx 20-25kDa band in Human Skeletal Muscle lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 14kDa according to NP_002613. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Prefoldin
Molecular Weight	14.2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti PFDN1 antibody, anti PDF antibody, anti PFD1 antibody, anti prefoldin 1 antibody, anti prefoldin subunit 1 antibody

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Western blot analysis of K562 lysate using Prefoldin antibody

Quick Database Links

Gene Symbol

Prefoldin

NCBI Gene ID

5201

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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