PTCH (C Terminus) Antibody

SKU: orb125007

Description

Goat polyclonal antibody to PTCH1

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC
Dilution Range	ELISA: 1:16000, IHC-P: 8 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	IHC: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PTCH (C Terminus)
Protein Sequence	ELQDVECEERPR
Molecular Weight	161; 161; 154; 144
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti PTCH antibody, anti patched homolog (Drosophila) antibody, anti HGNC:9585 antibody, anti BCNS antibody, anti HPE7 antibody, anti NBCCS antibody, anti PTC antibody, anti PTC1 antibody, anti patched antibody, anti patched (Drosophila) homolog antibody, anti PTCH1 antibody, anti patched homolog 1 (Drosophila) antibody, anti RP11-435O5.3 antibody, anti FLJ26746 antibody, anti FLJ42602 antibody, anti PTCH11 antibody, anti OTTHUMP00000021709 antibody, anti OTTHUMP00000021710 antibody, anti PTCH protein +12b antibody, anti PTCH protein +4 antibody, anti PTCH protein-10 antibody

Quality Guarantee

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orb125007 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of HeLa cells using PTCH1 antibody

orb125007 (3.8µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of NIH3T3 cells using PTCH1 antibody

Quick Database Links

Gene Symbol

PTCH (C Terminus)

NCBI Gene ID

5727

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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