RANGAP1 Antibody

SKU: orb18635

Description

Goat polyclonal antibody to RANGAP1

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 2-4 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml.WB: Approx 70kDa band observed in Human Brain and 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	RANGAP1
Molecular Weight	63.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti RANGAP1 antibody, anti KIAA1835 antibody, anti Ran GTPase activating protein 1 antibody, anti Fug1 antibody, anti MGC20266 antibody, anti SD antibody, anti OTTHUMP00000198756 antibody, anti OTTHUMP00000198757 antibody, anti segregation distorter homolog antibody, anti segregation distortion antibody

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orb18635 staining (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18635 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RANGAP1

NCBI Gene ID

5905

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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