SEPT6 Goat Polyclonal Antibody

SKU: orb20255

Description

This product is a goat polyclonal antibody targeting SEPT6, supplied unconjugated at 0.5 mg/ml. Validated for multiple applications including ELISA, flow cytometry, immunofluorescence, immunohistochemistry, and Western blot, it exhibits reactivity with human samples and shows predicted reactivity with bovine and canine species.

Research Area

Stem Cell & Developmental Biology; Cell Biology

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml. Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Reactivity	Human
Predicted Reactivity	Bovine, Canine

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.
Target	SEPT6
Protein Sequence	DEVNAFKQRKTA
Molecular Weight	48.9 kDa; 49.7 kDa; 49.2 kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti SEPT6 antibody, anti septin 6 antibody, anti KIAA0128 antibody, anti MGC16619 antibody, anti MGC20339 antibody, anti SEP2 antibody, anti SEPT2 antibody, anti septin 2 antibody

Quality Guarantee

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Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Primary incubation 1 hour at room temperature. Image A: Human Testes lysate at primary Ab concentration 1 ug/ml, Image B: Human Duodenum lysate at primary Ab concentration 0.1 ug/ml, Image C: Human Kidney lysate at primary Ab concentration 0.5 ug/ml, Image D: Human Tonsil lysate at primary Ab concentration 0.3 ug/ml (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Primary incubation 1 hour at room temperature. Images A, B, C: Daudi, Jurkat, MOLT-4 cell lysates at primary Ab concentration 0.1 ug/ml (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

3.8 µg/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).