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SIRT4 Antibody

SKU: orb18632

Description

Goat polyclonal antibody to SIRT4

Research Area

Cancer, Cardiovascular Disease, DNA Damage

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-3 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3ug/ml.WB: Approx. 38kDa band observed in Human Kidney and U937 lysates (calculated MW of 35.2kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.

Key Properties

ClonalityPolyclonal
TargetSIRT4
Protein SequenceSRCGELLPLIDPC
Molecular Weight35.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti SIRT4 antibody, anti SIR2L4 antibody, anti sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody, anti sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae) antibody, anti MGC130046 antibody, anti MGC130047 antibody, anti MGC57437 antibody, anti SIR2L4 antibody, anti sirtuin 4 antibody, anti sir2-like 4 antibody, anti sirtuin 4 antibody, anti sirtuin type 4 antibody

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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