SLC6A4/5HTT/SERT Antibody

SKU: orb19053

Description

Goat polyclonal antibody to Serotonin Transporter

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, WB
Dilution range	ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70-75kDa bands observed in Human Duodenum lysates (calculated MW of 70.3kDa according to NP_001036.1). Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	SLC6A4 / 5HTT / SERT
Molecular Weight	70.3
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti Serotonin transporter antibody, anti 5HT transporter antibody, anti 5HTT antibody, anti hSERT antibody, anti HTT antibody, anti OCD1 antibody, anti SERT antibody, anti SERT1 antibody, anti SERT antibody, anti SLC6A4 antibody, anti Sodium-dependent serotonin transporter antibody, anti Solute carrier family 6 member 4 antibody

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orb19053 staining (1ug/ml) of Human Duodenum lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells using Serotonin Transporter antibody.

Quick Database Links

Gene Symbol

SLC6A4 / 5HTT / SERT

NCBI Gene ID

6532

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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