Tankyrase 2 Antibody

SKU: orb19998

Description

Goat polyclonal to Tankyrase 2

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:16000, WB: 0.5-1.5 μg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 125 kDa band observed in lysates of cell line Jurkat (calculated MW of 127 kDa according to NP_079511.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Tankyrase 2
Molecular Weight	127
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti TNKS2 antibody, anti tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 antibody, anti PARP-5b antibody, anti PARP-5c antibody, anti PARP5B antibody, anti PARP5C antibody, anti TANK2 antibody, anti TNKL antibody, anti tankyrase 2 antibody

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orb19998 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Tankyrase 2

NCBI Gene ID

80351

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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