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Thrombospondin Antibody

SKU: orb167695

Description

Goat polyclonal antibody to THBS1.

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution rangeELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine, Rat
Application Notes
WB: Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Key Properties

HostGoat
ClonalityPolyclonal
TargetThrombospondin
Molecular Weight129
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

Anti THBS1 antibody, anti thrombospondin 1 antibody, anti THBS antibody, anti TSP antibody, anti TSP1 antibody, anti thrombospondin-1p180 antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Thrombospondin Antibody

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) using THBS1 antibody

Thrombospondin Antibody

Immunohistochemical staining of Human Spleen using THBS1 antibody

Thrombospondin Antibody

Immunohistochemical staining of Human Placenta using THBS1 antibody

Thrombospondin Antibody

Western blot analysis of Mouse Kidney lysate using THBS1 antibody

Thrombospondin Antibody

Immunohistochemical staining of Human Spleen using THBS1 antibody

Thrombospondin Antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using THBS1 antibody

Thrombospondin Antibody

Immunohistochemical staining of Human Spleen using THBS1 antibody

Thrombospondin Antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells using THBS1 antibody

Thrombospondin Antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells using THBS1 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Thrombospondin Antibody (orb167695)

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Available Sizes

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100 μg
¥ 6,500.00
DispatchUsually dispatched within 5-10 working days
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