TMP21/TMED10 Antibody

SKU: orb19451

Description

Goat polyclonal antibody to TMED10

Research Area

Cancer, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, IF
Dilution Range	ELISA: 1:1000, WB: 1 μg/ml, IHC-P: 10ug/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments gave an approx 75kDa band in Human and Rat Pancreas lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	TMP21 / TMED10
Protein Sequence	YLRRFFKAKKLIE
Molecular Weight	25
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti TMED10 antibody, anti transmembrane emp24-like trafficking protein 10 (yeast) antibody, anti P24(DELTA) antibody, anti S31I125 antibody, anti S31III125 antibody, anti TMP21 antibody, anti Tmp-21-I antibody, anti 21 kDa transmembrane trafficking protein antibody, anti transmembrane emp24 domain-containing protein 10 antibody

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Immunofluorescence analysis of U251 cells using TMED10 antibody

Flow cytometric analysis of HeLa cells using TMED10 antibody

Quick Database Links

Gene Symbol

TMP21 / TMED10

NCBI Gene ID

10972

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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