Vimentin Antibody

SKU: orb233645

Description

Goat polyclonal antibody to Vimentin

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution range	ELISA: 1:4000, WB: 0.1-2 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	WB: Approx 55kDa band observed in lysates of cell line HeLa and Jurkat. Recommended concentration: 1-3 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Vimentin
Molecular Weight	53.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Disclaimer	For research use only

Alternative Names

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anti CTRCT30 antibody, anti Epididymis luminal protein 113 antibody, anti HEL113 antibody, anti VIM antibody, anti Vimentin antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

orb233645 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb233645 (0.1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using Vimentin antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using Vimentin antibody.

Immunohistochemical staining of Human Kidney using Vimentin antibody.

Quick Database Links

Gene Symbol

Vimentin

NCBI Gene ID

7431

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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