VPS29 (Internal) Antibody

SKU: orb19270

Description

Goat polyclonal antibody to VPS29

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC
Dilution Range	ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	VPS29 (Internal)
Protein Sequence	CTGNLCTKESYDYLK
Molecular Weight	20.5; 20.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti VPS29 antibody, anti vacuolar protein sorting 29 (yeast) antibody, anti DC15 antibody, anti DC7 antibody, anti DKFZp564F0223 antibody, anti FLJ20492 antibody, anti PEP11 antibody, anti retromer protein antibody, anti vacuolar protein sorting 29 antibody, anti vacuolar protein sorting 29 (yeast homolog) antibody, anti vacuolar sorting protein VPS29/PEP11 antibody, anti x 007 protein antibody

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Immunohistochemical staining of Human Uterus using VPS29 antibody

Quick Database Links

Gene Symbol

VPS29 (Internal)

NCBI Gene ID

51699

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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