Goat Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit

SKU: orb779818

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic(FGF1).

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Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat FGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat FGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat FGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat FGF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Goat
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	32 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ECGF ELISA Kit, AFGF ELISA Kit, ECGFA ELISA Kit, ECGFB ELISA Kit, FGF-Alpha ELISA Kit, FGFA ELISA Kit, HBGF1 ELISA Kit, ECGFB ELISA Kit, HBGF1 ELISA Kit, Acidic Fibroblast Growth Factor ELISA Kit, heparin-binding growth factor 1 ELISA Kit, Endothelial Cell Growth Factor, Beta ELISA Kit

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Goat Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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