Goat IgG (H&L) Secondary Antibody Fluorescein Conjugated

SKU: orb346985

Description

Goat IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Goat
Application Notes	Anti-Goat IgG Fluorescein Antibody has been tested by dot blot and western blot. Secondary antibody reagents are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Goat IgG whole molecule
Purity	Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.

Storage & Handling

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Storage	Store anti-goat FITC conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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FITC Conjugated Secondary Antibody, Anti-Goat FITC, rabbit anti-goat IgG fluorescein conjugated antibody

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Goat IgG (H&L) Secondary Antibody Fluorescein Conjugated

Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated. Lane 1: Goat IgG. Load: 50 ng per lane. Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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DOT

Dot Blot

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