Golimumab ELISA Kit

SKU: orb2975827

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human TNFa has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Golimumab in the sample competitively binds to the pre-coated protein with biotin-labeled Golimumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Golimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Images & Validation

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Key Properties

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Sample Types	Plasma, Serum
Assay Type	Quantitative
Range	62.5 - 4,000 ng/mL
Sensitivity	40.1 ng/mL

Storage & Handling

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Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.
Disclaimer	For research use only

Alternative Names

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CNTO 148, CAS: 476181-74-5

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