Gramicidin

SKU: orb1309668

Description

Gramicidin is an antimicrobial peptide that forms ion channels in cell membranes, disrupting ionic gradients and killing cells. It is widely used in research on membrane biophysics, ion transport mechanisms, and as a tool compound in both in vitro and in vivo studies of bacterial cytotoxicity and channel function.

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Key Properties

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CAS Number	1405-97-6
MW	1882.33
Purity	97.03% (May vary between batches)
Formula	C₉₉H₁₄₀N₂₀O₁₇
SMILES	C[C@@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC3=CNC4=CC=CC=C43)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC5=CNC6=CC=CC=C65)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC7=CNC8=CC=CC=C87)C(=O)NCCO)NC(=O)CNC(=O)[C@H](C(C)C)NC=O
Target	Antibacterial,Antibiotic,MRP
Solubility	DMSO:50 mg/mL (26.56 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (1.06 mM)

Bioactivity

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In Vivo

METHODS: To study the effect on in vivo allograft survival, Gramicidin (2-4 mg/kg) was injected intraperitoneally into ectopic heart transplanted rats once daily for 6 days. RESULTS: Graft pulsation in the control group ceased at 5.4+/-0.4 days post-transplantation due to acute allograft rejection, whereas recipients in the group receiving intraperitoneal injections of Gramicidin (2-4 mg/kg) had a significant prolongation of graft pulsation to 15.4+/-4.3 or 18.4+/-3.9 days post-transplantation, respectively.

In Vitro

METHODS: OC cells OVCAR8, A2780 and SKOV3 were treated with Gramicidin (0.33-3 µM) for 24-72 h. Cell viability was measured by WST-1 assay. RESULTS: Gramicidin inhibited the growth of OC cells in a dose- and time-dependent manner, with IC50 values of 0.0763, 0.1856 and 0.1148 µM for OVCAR8, SKOV3 and A2780 cells, respectively, at 72 h. METHODS: Human gastric cancer cells SGC-7901 were treated with Gramicidin (0.3-3 µM) for 24-48 h. Apoptosis was detected by Flow cytometry. RESULTS: Gramicidin promoted apoptosis in SGC-7901 cells in a time- and dose-dependent manner. When cells were incubated with Gramicidin for 48 h, the percentage of viable cells decreased to 54.2% with 3 µM Gramicidin treatment compared to control.

Storage & Handling

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Storage	keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Antibiotic, Bacterial, MRP1, inhibit, Inhibitor, Gramicidin, Gramicidina, Gramicidine, Gramicidinum

Quality Guarantee

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Compound Identifiers

PubChem CID

16130140 ↗

Key Properties

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No computed properties available.

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Gramicidin (orb1309668)

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CAS Number:	1404-88-2
MW:	1228.459
Formula:	C₆₅H₈₅N₁₁O₁₃

CAS Number:	1257-08-5
MW:	442.37
Formula:	C₂₂H₁₈O₁₀

CAS Number:	11029-61-1
MW:	1882.29
Formula:	C₉₉H₁₄₀N₂₀O₁₇

Gramicidin

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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