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GST Antibody (HRP)
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | WB (1:1000); ELISA (1:800) |
| Reactivity | All |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 300 |
| Immunogen | Glutathione-S-Transferase (GST) tagged protein |
| Target | GST |
| Molecular Weight | 26 kDa |
| Purification | Protein G Purified |
| Conjugation | HRP |
Storage & Handling
−| Storage | Conjugated antibodies should be stored according to the product label |
|---|---|
| Buffer/Preservatives | 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Western Blot analysis of Schistosoma japonicum Purified GST showing detection of 26 kDa GST protein using Mouse Anti-GST Monoclonal Antibody, Clone 3E2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein. Lane 3: GST. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GST Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 26 kDa.
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Protocol Information
GST Antibody (HRP) (orb413652)
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