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GST Antibody (HRP)

SKU: orb413652

Description

Mouse monoclonal antibody against GST conjugated to HRP

Research Area

Cell Biology, Disease Biomarkers, Metabolism Research, Signal Transduction

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeWB (1:1000); ELISA (1:800)
ReactivityAll
Application Notes
A 1:1000 dilution of SMC-549 was sufficient for detection of GST in 1 µg of Purified GST tagged PmtA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.300
ImmunogenGlutathione-S-Transferase (GST) tagged protein
TargetGST
Molecular Weight26 kDa
PurificationProtein G Purified
ConjugationHRP

Storage & Handling

StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Glutathione S Transferase, Glutathione S-transferase class-mu 26 kDa isozyme, GST, Sj26 antigen, SjGST, Glutathione-S-Transferase, GST tag, GST tagged protein, GST fusion protein, GST protein

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GST Antibody (HRP)

Western Blot analysis of Schistosoma japonicum Purified GST showing detection of 26 kDa GST protein using Mouse Anti-GST Monoclonal Antibody, Clone 3E2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein. Lane 3: GST. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GST Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 26 kDa.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GST Antibody (HRP) (orb413652)

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Available Sizes

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100 μg
¥ 8,320.00
DispatchUsually dispatched within 2-3 weeks
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