GTPBP9 Rabbit Polyclonal Antibody

SKU: orb2897

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Description

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Item 1 of 4

Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Drosophila, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GTPBP9 (151-250/396aa)
Target	OLA1
Molecular Weight	45 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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DNA damage-regulated overexpressed in cancer 45 protein; DOC45; GBP45; GTP-binding protein 9(putative); GTP-binding protein 9; GTP-binding protein PTD004; homologous yeast-44.2 protein; Obg like ATPase 1; Obg-like ATPase 1; OLA1; OLA1_HUMAN; PTD004.

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Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody (orb2897), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-GTPBP9 (orb2897) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 50kD.

Sample: HepG2 Cell (Human) Lysate at 30 ug, TT Cell (Human) Lysate at 30 ug, Primary: Anti-GTPBP9 (orb2897) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 50kD.

Sample: Raji Cell (Human) Lysate at 30 ug, Primary: Anti-GTPBP9 (orb2897) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 50kD.

Quick Database Links

Gene Symbol

OLA1

UniProt

Q9NTK5

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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