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Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated

SKU: orb347067

Description

Guinea Pig IgG F(c) antibody (Alkaline Phosphatase)

Research Area

Neuroscience

Images & Validation

Tested ApplicationsDOT, ELISA, IHC, WB
Dilution RangeELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
ReactivityGuinea pig
Application Notes
Anti-Guinea Pig IgG F(c) Alkaline Phosphatase has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenGuinea Pig IgG F(c) fragment
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab).
ConjugationAP

Storage & Handling

StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

goat Anti-Guinea Pig IgG F(c) Antibody Alkaline Phosphatase Conjugated, goat Anti-Guinea Pig IgG Fc Fragment Antibody alk phos Conjugated

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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DOT
Dot Blot
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Available Sizes

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1 mg
$ 500.00
Free Secondary Antibody (20 ul)0/0

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