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Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated
SKU: orb347084
Description
Images & Validation
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:100,000, IHC: 1:500 - 2,500, WB: 1:2,000 - 10,000 |
| Reactivity | Guinea pig |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Guinea Pig IgG was produced by repeated immunization with guinea pig IgG whole molecule in rabbit. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 2.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Guinea Pig IgG (H&L) Antibody, Rb-a-Guinea Pig IgG(H&L) Peroxidase conjugated, Guinea Pig IgG(H&L) Peroxidase Antibody in rabbit, Rabbit IgG (H&L) peroxidase conjugated Secondary Antibody.
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Western Blot of Peroxidase conjugated Rabbit Anti-Guinea Pig IgG secondary antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated (orb347084)
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