HA tag Rabbit Polyclonal Antibody

SKU: orb1294277

Description

Rabbit polyclonal antibody to HA tag

Images & Validation

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Item 1 of 1

Tested Applications	IP, WB
Dilution Range	Western Blot 1:3000-1:7000, Immunofluorescence 1:500-1:1000

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide (YPYDVPDYA)
Target	HA tag
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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All lanes: Anti HA-tag antibody at 1/5000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294277 overnight at 4°C.

Quick Database Links

Gene Symbol

HA tag

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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