HA14-1

SKU: orb1306869

Description

HA14-1 is a cell-permeable, non-peptidic small molecule antagonist of the anti-apoptotic proteins Bcl-2 and Bcl-XL, binding to a surface pocket with an IC50 of approximately 9 µM. It is utilized in apoptosis research to investigate protein interactions and has been applied in both in vitro cell-based assays and in vivo xenograft studies to probe cancer biology.

Research Area

Cell Biology

Images & Validation

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Key Properties

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CAS Number	65673-63-4
MW	409.23
Purity	98.00% (May vary between batches)
Formula	C₁₇H₁₇BrN₂O₅
SMILES	CCOC(=O)C(C#N)C1C(C(=O)OCC)=C(N)Oc2ccc(Br)cc12
Target	Bcl-2 Family
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.89 mM);Ethanol:40.9 mg/mL (99.94 mM);DMSO:40.9 mg/mL (99.94 mM)

Bioactivity

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Target IC50	Bcl-2:9 μM
In Vivo	When applied to follicular lymphoma cell lines HF1A3, HF4.9, and HF28RA, HA14-1 exhibits cytotoxicity with LC50 values of 4.5/12.6/8.1 μM, respectively. HA14-1 induces apoptosis in HL-60 cells in a dose-dependent manner through the activation of caspases. Moreover, HA14-1 triggers apoptosis in HF4.9, HF1A3, and HF28RA cells through caspase and ROS (Reactive Oxygen Species) pathways. Read more...
In Vitro	In immunodeficient mice, HA14-1 (400 nM) does not significantly affect the growth of glioblastomas on its own. However, in vivo, HA14-1 (400 nM) enhances the efficacy of the DNA-damaging agent etoposide (2.5 mg/kg) in inhibiting the growth of glioblastomas. Read more...
Cell Research	The cytotoxic effects of HA14-1 against different FL cell lines are determined by the MTT assay. Briefly, the cells (5000/well) are incubated in triplicate in 96-well plate in the presence or absence of HA14-1 for 20 h at 37 °C. Thereafter, the MTT solution is added to each well. After 4 h incubation at 37 °C, the optical density (OD) is measured by means of 96-well plate reader, with the extraction buffer as a blank. The following formula is used: percentage cell viability = (OD of the experiment samples/OD of the control) × 100. Sigmoidal dose-response curves are fitted to the mean cell viability plotted against log HA14-1 dose and lethal concentration 50% (LC50) values are calculated from the resulting curves using Prism 4.0 softw(Only for Reference) Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HA14 1, HA141, HA-14-1, HA14-1, Inhibitor, inhibit, Bcl-2 Family, Bcl-2

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Compound Identifiers

PubChem CID

3549 ↗

Key Properties

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No computed properties available.

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HA14-1 (orb1306869)

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Purity:	>98% (HPLC)
CAS Number:	65673-63-4
MW:	409.2313
Formula:	C₁₇H₁₇BrN₂O₅

HA14-1

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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