HADHA Antibody (C-term) [Knockout Validated]

HADHA Antibody (C-term) western blot analysis in A431 cell line lysates (35 ug/lane). This demonstrates the HADHA antibody detected the HADHA protein (arrow).

HADHA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-HADHA Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

A predominant 75 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti-HADHA) vs the predicted size of 83 kDa. The molecular weight discrepancy could be due to post-translationally modification of the target protein, a splice-variant form of the target protein, a partially degraded form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A less intense band at 65 kDa was also A weak band only in the 20ug lane was observed for the knock out lysate, suggesting incomplete knockout of the target gene.