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HBB Antibody

SKU: orb420234

Description

Hemoglobin A (beta chain) antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:20,000, WB: 1ug/mL
ReactivityHuman
Application Notes
Anti-Hemoglobin A (beta chain) (MOUSE) antibody has been tested by ELISA, SDS-Page, and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
Clone No.14G2.G11.F11
ImmunogenAnti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
TargetHBB
PurityThis protein A purified mouse monoclonal antibody reacts specifically with human HbA beta chain isoform. Anti-Hemoglobin beta β is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbS, HbF (gamma), or HbC forms. HbA antibody cross reacts with HbA-2.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.04 mg/ml by UV absorbance at 280 nm
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

mouse anti-HbA antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit, Hemoglobin subunit beta, Hemoglobin beta chain, HBB, HBA, Hbβ Antibody, LVV-hemorphin-7, Spinorphin, Sickle Cell Disease (SCD)

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 910.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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