Recombinant HER-2 Antibody / ErbB2

SKU: orb2636504

Description

C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant HER-2 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2b, kappa
Clone No.	rERBB2/9401
Immunogen	A recombinant fragment (within amino acids 1155-1255) of human HER-2/ERBB2 protein was used as the immunogen for the recombinant HER-2 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human breast carcinoma tissue with recombinant HER-2 antibody (clone rERBB2/9401). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant HER-2 antibody (clone rERBB2/9401) as confirmation of integrity and purity.

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UniProt

P04626

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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