HEY1 Rabbit Polyclonal Antibody

SKU: orb574242

Description

Rabbit polyclonal antibody to HEY1

Research Area

Cancer Biology, Epigenetics & Chromatin, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 8

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the C terminal region of human HEY1
Target	HEY1
Protein Sequence	Synthetic peptide located within the following region: PQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSFGPVLPVVTS
Molecular Weight	33 kDa
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	1.0 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CHF2, OAF1, HERP2, HESR1, HRT-1, NERP2, hHRT1, BHLHb31

Quality Guarantee

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Sample Tissue: Human 293T Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human 293T, Antibody dilution: 1.0 ug/ml.

Sample Tissue: Human ACHN Whole Cell, Antibody dilution: 3 ug/ml.

Positive control (+): NCI-H226 (N13), Negative control (-): U937 (N31), Antibody concentration: 1 ug/ml.

Rabbit Anti-HEY1 Antibody, Paraffin Embedded Tissue: Human Kidney, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

Rabbit Anti-HEY1 Antibody, Paraffin Embedded Tissue: Human Lung, Cellular Data: Alveolar cells, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-HEY1 Antibody, Titration: 2.5 ug/ml, Positive Control: Lung.

Quick Database Links

Gene Symbol

HEY1

UniProt

Q9Y5J3

RefSeq (Protein):NP_036390

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_036390

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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