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HHLA2 Antibody
SKU: orb1270692
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human HHLA2. |
| Target | HHLA2 |
| Molecular Weight | 47 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2
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Western Blot at 1:2000 dilution Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
HHLA2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB453, CEM, HL-60, MDA-MB231 cell line lysates (35 ug/lane).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
HHLA2 Antibody (orb1270692)
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