HIF1 alpha Rabbit pAb

SKU: orb1563004

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Description

Research Area

Cardiovascular Research

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human HIF-1alpha.
Target	HIF1A
Molecular Weight	Calculated MW: 93 kDa; Observed MW: 92-130 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Disclaimer	For research use only

Alternative Names

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HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma

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Western blot analysis of HIF1 alpha in LOVO lysates using HIF1 alpha antibody.

Immunohistochemistry analysis of paraffin-embedded mouse colon tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded rat lung tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Quick Database Links

Gene Symbol

HIF1A

UniProt

Q16665

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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