Histone H3 / H3-3A antibody

SKU: orb2276429

Description

Histone H3 / H3-3A antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution range	1:100-1:200
Reactivity	Human
Application Notes	Positive Control: Lymph node: A strong nuclear Histone H3 staining should be seen in all cells while strongest staining intensity occurs in cells of germinal centres. Negative Control: Colon, Kidney: Histone H3 staining should be seen in nuclei only. Cytoplasmic staining should not be seen. Cellular Localization: Intracelullar Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	MSVA-903M
Immunogen	Recombinant human H3.3 histone A fragment
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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H3.3 Histone A, H3.3A, H3F3A, H3F3, H3 Histone Family Member 3A, H3 Histone, Family 3A, Histone H3.3, BRYLIB1, H3- 3B, H3.3B, H3F3B

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Clear cell renal cell carcinoma with strong Histone H3 staining of tumor cells.

Colon mucosa with distinct nuclear Histone H3 staining.

Kidney with Histone H3 staining of nuclei.

Quick Database Links

UniProt

P84243

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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