Histone H3 K4me2/phospho T6 Antibody

SKU: orb420434

Description

Histone H3 K4me2 antibody

Images & Validation

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Tested Applications	ChIP, DOT, ELISA, IF, IHC, IP, WB
Dilution range	ELISA: 1: 10,000, IHC: 1:50, IF: 1:50, IP: 1:100, WB: 1:500
Reactivity	C. elegans, Human, Mouse, Rat
Application Notes	Anti-Histone H3 [Dimethyl Lys4/pT6] antibody is tested for CHIP, immunocytochemistry, dot blot, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Histone H3 [Dimethyl Lys4/pT6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K4me2/pT6 site of human Histone H3.
Purity	Anti-Histone H3 [Dimethyl Lys4/pT6] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Dimethyl Lys4/pT6] from other sources has not been determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.31 mg/mL
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-Histone H3 dimethyl Lys4 pT6 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K4me2pT6

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